2026

Biotechnology

Biotech Genetic Engineering: Expert Hard Challenge

Nine tough questions dissect advanced genetic engineering concepts, from precise DNA cutting to seamless fragment assembly.

#recombinant DNA#restriction enzymes#CRISPR-Cas9#selectable markers#Gibson assembly#Cre-loxP#BACs#plasmids#gene cloning

Quiz Intelligence

Total Questions

9

Difficulty Level

hard

Progress

0/0

0%

Quiz Questions

Answer all questions below and test your knowledge.

1
Which enzyme class produces sticky ends by cleaving DNA at specific recognition sequences in cloning?
Question 1
2
In the CRISPR-Cas9 system, the PAM sequence is essential because it:
Question 2
3
Bacterial Artificial Chromosomes (BACs) excel in cloning large DNA fragments primarily due to their:
Question 3
4
The Cre-loxP system facilitates:
Question 4
5
Gibson assembly enables seamless joining of DNA fragments through a combination of:
Question 5
6
The key distinction of shRNA from siRNA in gene silencing is that shRNA:
Question 6
7
Yeast Artificial Chromosomes (YACs) are ideal for very large inserts because they stably maintain up to:
Question 7
8
Selectable markers in genetic engineering confer:
Question 8
9
TALENs differ from zinc finger nucleases in that their DNA-binding domains consist of:
Question 9

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Biotech Genetic Engineering: Expert Hard Challenge

Quiz Intelligence

Quiz Questions

Which enzyme class produces sticky ends by cleaving DNA at specific recognition sequences in cloning?

In the CRISPR-Cas9 system, the PAM sequence is essential because it:

Bacterial Artificial Chromosomes (BACs) excel in cloning large DNA fragments primarily due to their:

The Cre-loxP system facilitates:

Gibson assembly enables seamless joining of DNA fragments through a combination of:

The key distinction of shRNA from siRNA in gene silencing is that shRNA:

Yeast Artificial Chromosomes (YACs) are ideal for very large inserts because they stably maintain up to:

Selectable markers in genetic engineering confer:

TALENs differ from zinc finger nucleases in that their DNA-binding domains consist of: